2VYT
The MBT repeats of human SCML2 bind to peptides containing mono methylated lysine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.530, 99.210, 131.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.2167 |
| Rwork | 0.217 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oi1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.444 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.320 |
| Number of reflections | 40544 | |
| <I/σ(I)> | 13.2 | 3.8 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 0.1M CHES, PH 9.5 40% PEG 600 5MM MONOMETHYLATD LYSINE 10MM MBT |
