2VYC

Crystal Structure of Acid Induced Arginine Decarboxylase from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	77
Detector technology	CCD
Collection date	2006-12-07
Detector	ADSC CCD
Spacegroup name	P 64
Unit cell lengths	197.651, 197.651, 450.322
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.179
Rwork	0.177
R-free	0.22900
Structure solution method	MIRAS
Starting model (for MR)	NONE
RMSD bond length	0.013
RMSD bond angle	1.345
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.150	0.780
Number of reflections	385411
<I/σ(I)>	10.7	2.3
Completeness [%]	99.9	100
Redundancy	5	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		100 MM MES PH 6.5, 13% PEG 8000 AND 400 MM SODIUM ACETATE
1		6.5		100 MM MES PH 6.5, 13% PEG 8000 AND 400 MM SODIUM ACETATE
1		6.5		100 MM MES PH 6.5, 13% PEG 8000 AND 400 MM SODIUM ACETATE