2VY8
The 627-domain from influenza A virus polymerase PB2 subunit with Glu- 627
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-10 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 48.500, 45.700, 60.840 |
| Unit cell angles | 90.00, 106.24, 90.00 |
Refinement procedure
| Resolution | 29.210 - 1.200 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 627-DOMAIN WITH K627 FROM PDB ENTRY 2VY7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.537 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.200 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.100 | 0.330 |
| Number of reflections | 42201 | |
| <I/σ(I)> | 5.07 | 2.5 |
| Completeness [%] | 92.9 | 85.9 |
| Redundancy | 2.84 | 1.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1 MICROLITRE OF 2.4 MG/ML PROTEIN SOLUTION IN 10 MM TRIS-HCL PH 7.0 AND 200 MM NACL WITH 1 MICROLITRE OF 100 MM CITRIC ACID PH 4.0-7.0 AND 1.4-1.6 M AMMONIUM SULFATE SOLUTION |
