2VXS
Structure of IL-17A in complex with a potent, fully human neutralising antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.473, 66.708, 203.790 |
| Unit cell angles | 90.00, 91.65, 90.00 |
Refinement procedure
| Resolution | 204.120 - 2.630 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aqk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.361 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0036) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.100 | 2.770 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.130 | 0.460 |
| Number of reflections | 79235 | |
| <I/σ(I)> | 11.7 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 90.9 MM PCTP BUFFER PH 4.0, 9.1 MM PCTP BUFFER PH 9.5, 100 MM AMMONIUM SULPHATE, 14% W/V PEG3350 |
