2VX2
Crystal structure of human enoyl Coenzyme A hydratase domain- containing protein 3 (ECHDC3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-10 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 235.756, 135.296, 83.871 |
| Unit cell angles | 90.00, 98.84, 90.00 |
Refinement procedure
| Resolution | 117.040 - 2.300 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uiy |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.633 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.000 | 2.460 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.520 |
| Number of reflections | 118152 | |
| <I/σ(I)> | 9.7 | 2.3 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.20M NA(FORM); 0.1M BTPROP PH 8.5, 20.0% PEG350, 10.0% ETGLY |
