Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-14 |
Detector | ADSC CCD |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 73.805, 73.805, 110.667 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.720 - 2.400 |
R-factor | 0.198 |
Rwork | 0.195 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1amw |
RMSD bond length | 0.011 |
RMSD bond angle | 1.444 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.060 | 0.340 |
Number of reflections | 12582 | |
<I/σ(I)> | 20.2 | 5.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.8 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7 |