2VVP
Crystal structure of Mycobacterium tuberculosis ribose-5-phosphate isomerase B in complex with its substrates ribose 5-phosphate and ribulose 5-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-05-05 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.832, 103.058, 69.728 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 34.710 - 1.650 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1usl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.437 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC (5) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.290 |
| Number of reflections | 115358 | |
| <I/σ(I)> | 13.3 | 3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3000, 0.1 M TRIS PH 7, 0.2 M CA ACETATE |
