2VVI
IrisFP fluorescent protein in its green form, trans conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.310, 96.380, 140.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.570 - 2.000 |
| R-factor | 0.1961 |
| Rwork | 0.193 |
| R-free | 0.25138 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zux |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.365 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.470 |
| Number of reflections | 73301 | |
| <I/σ(I)> | 10.1 | 2.8 |
| Completeness [%] | 97.3 | 98.6 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.4 | 293 | CRYSTALLIZATION CONDITIONS: AMMONIUM SULFATE, BICINE, PH 8.4, VAPOR DIFFUSION, TEMPERATURE 293K |
