2VVE
Crystal structure of the stem and receptor binding domain of the spike protein P1 from bacteriophage PM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 |
| Unit cell lengths | 107.894, 107.894, 44.171 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.000 - 1.770 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vvd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.097 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.080 | 0.870 |
| Number of reflections | 47548 | |
| <I/σ(I)> | 30.2 | 1.8 |
| Completeness [%] | 94.9 | 71.8 |
| Redundancy | 11.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 7.3MG/ML (IN 150MM NACL, 20MM TRIS PH 7.5, 10MM CACL2),20%(W/V) PEG6K,100MM TRIS PH 8, 200MM LICL |
