2VUX
Human ribonucleotide reductase, subunit M2 B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.960, 99.470, 132.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 2.800 |
| R-factor | 0.213 |
| Rwork | 0.209 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uw2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.138 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.660 |
| Number of reflections | 22880 | |
| <I/σ(I)> | 13.99 | 2.81 |
| Completeness [%] | 98.1 | 99.4 |
| Redundancy | 3.59 | 3.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M NACACODYLATE PH 6.5, 2.3M AMMONIUM SULFATE, 0.2M SODIUM CHLORIDE. CHYMOTRYPSIN WAS ADDED TO A RATIO CHYMOTRYPSIN:PROTEIN OF 1:100 PRIOR TO CRYSTALLISATION |
