2VUG
The structure of an archaeal homodimeric RNA ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2006-07-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.962, 103.257, 150.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.227 |
| Rwork | 0.224 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.273 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.100 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.130 | 0.690 |
| Number of reflections | 20985 | |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 50% METHANE PENTANE-DIOL (MPD), 0-0.2M NH4H2PO4 AND 0.1M TRIS-CL, PH 8.5 |
