2VT0
X-ray structure of a conjugate with conduritol-beta-epoxide of acid-beta-glucosidase overexpressed in cultured plant cells
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-03 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.184, 96.884, 83.171 |
| Unit cell angles | 90.00, 103.73, 90.00 |
Refinement procedure
| Resolution | 19.770 - 2.150 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v3f |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.993 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.410 |
| Number of reflections | 59355 | |
| <I/σ(I)> | 10.4 | 1.73 |
| Completeness [%] | 97.1 | 82.7 |
| Redundancy | 3.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 0.2M AMMONIUM SULFATE; 0.1M TRIS PH 6.5; 25% W/V PEG 3350; CRYSTALLIZATION UNDER OIL |
