2VS4
THE BINDING OF UDP-GALACTOSE BY AN ACTIVE SITE MUTANT OF alpha-1,3 GALACTOSYLTRANSFERASE (alpha3GT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.251, 94.630, 94.690 |
| Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
| Resolution | 32.500 - 1.770 |
| R-factor | 0.263 |
| Rwork | 0.263 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4v |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.070 | 0.230 |
| Number of reflections | 76955 | |
| <I/σ(I)> | 20.4 | 3.6 |
| Completeness [%] | 92.3 | 87.5 |
| Redundancy | 5.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PROTEIN: 10 MM UDP-GAL, 10 MM MNCL2, 10 MM N-ACETYLLACTOSAMINE, MOTHER LIQUOR: 5-10 % PEG 6000, 0.1 M TRIS-HCL PH 8.0, 5-14 % MPD |
