2VS3
THE BINDING OF UDP-GALACTOSE BY AN ACTIVE SITE MUTANT OF alpha-1,3 GALACTOSYLTRANSFERASE (alpha3GT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.116, 94.380, 94.940 |
| Unit cell angles | 90.00, 99.30, 90.00 |
Refinement procedure
| Resolution | 35.180 - 2.200 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4v |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.120 | 0.310 |
| Number of reflections | 39855 | |
| <I/σ(I)> | 9.7 | 2.5 |
| Completeness [%] | 94.9 | 86.2 |
| Redundancy | 8.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PROTEIN: 10 MM UDP-GAL, 10 MM MNCL2, 10 MM N-ACETYLLACTOSAMINE, MOTHER LIQUOR: 5-10 % PEG 6000, 0.1 M TRIS-HCL PH 8.0, 5-14 % MPD |
