2VS1
The crystal structure of Pyrococcus abyssi tRNA (uracil-54, C5)- methyltransferase in complex with S-adenosyl-L-homocysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.529, 100.415, 55.737 |
| Unit cell angles | 90.00, 111.61, 90.00 |
Refinement procedure
| Resolution | 68.040 - 2.100 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.25100 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.342 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.400 |
| Number of reflections | 28990 | |
| <I/σ(I)> | 19 | 3 |
| Completeness [%] | 97.4 | 96.4 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15% PEG 8000, 0.05 M AMMONIUM SULFATE, 0.1 M SODIUM CITRATE PH 5.6 |
