2VRW
Critical structural role for the PH and C1 domains of the Vav1 exchange factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-05 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.763, 62.143, 103.281 |
| Unit cell angles | 90.00, 118.80, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.850 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.031 |
| Data reduction software | DEN |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.930 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 57653 | |
| <I/σ(I)> | 19.2 | 2.8 |
| Completeness [%] | 99.2 | 97.9 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 100MM BICINE PH9.0 10% PEG 6000 |
