2VRR
Structure of SUMO modified Ubc9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.520, 66.610, 122.579 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.310 - 2.220 |
| R-factor | 0.175 |
| Rwork | 0.171 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1U9B 2bf8 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.633 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.320 |
| High resolution limit [Å] | 2.220 | 2.210 |
| Rmerge | 0.140 | 0.430 |
| Number of reflections | 11764 | |
| <I/σ(I)> | 5.2 | 1.8 |
| Completeness [%] | 97.3 | 81.5 |
| Redundancy | 12.4 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 24% (W/V) PEG3350, 200 MM SODIUM FORMATE, 100 MM BIS-TRIS PROPANE PH6.5 |
