2VR5
Crystal structure of Trex from Sulfolobus Solfataricus in complex with acarbose intermediate and glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 97 |
| Detector technology | CCD |
| Collection date | 2007-11-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 204.814, 204.814, 89.558 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.850 - 2.800 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.26600 |
| Structure solution method | MIR |
| Starting model (for MR) | 2vnc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.600 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.090 | 0.270 |
| Number of reflections | 51526 | |
| <I/σ(I)> | 15.4 | 3.7 |
| Completeness [%] | 96.6 | 93 |
| Redundancy | 6.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PEG 3K 8%, 0.2M LITHIUM SULFATE, 0.1M PH 8.0 IMIDAZOLE BUFFER |
