2VQI
Structure of the P pilus usher (PapC) translocation pore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-07 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.500, 101.900, 113.700 |
| Unit cell angles | 90.00, 128.20, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.200 |
| R-factor | 0.2593 |
| Rwork | 0.259 |
| R-free | 0.29580 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.736 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.100 | 0.220 |
| Number of reflections | 48050 | |
| <I/σ(I)> | 9.7 | 5.5 |
| Completeness [%] | 98.4 | 99.3 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 5-20 % 3-METHYL-1,5-PENTANEDIOL (MPD), 6-12 % POLYETHYLENE GLYCOL 4000 (PEG4000), 50 MM NA CITRATE PH 5.6, 100 MM AMMONIUM SULFATE |
