2VPN
High-resolution structure of the periplasmic ectoine-binding protein from TeaABC TRAP-transporter of Halomonas elongata
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.300, 52.200, 63.500 |
| Unit cell angles | 80.80, 85.80, 77.90 |
Refinement procedure
| Resolution | 20.000 - 1.550 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.20400 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.291 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.010 | 0.060 |
| Number of reflections | 81024 | |
| <I/σ(I)> | 9.2 | 2.3 |
| Completeness [%] | 93.3 | 92.3 |
| Redundancy | 3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 0.1 M MGCL2 AND 25.5 % PEG 3350 |
