2VPJ
Crystal structure of the Kelch domain of human KLHL12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.637, 61.546, 45.523 |
| Unit cell angles | 90.00, 111.75, 90.00 |
Refinement procedure
| Resolution | 42.300 - 1.850 |
| R-factor | 0.164 |
| Rwork | 0.161 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dyh |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.571 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.300 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.090 | 0.630 |
| Number of reflections | 19527 | |
| <I/σ(I)> | 10.6 | 2 |
| Completeness [%] | 99.4 | 96.1 |
| Redundancy | 4.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.2M AMMONIUM ACETATE, 0.1M SODIUM ACETATE PH4.6, 30% PEG4K |
