2VPE
Decoding of methylated histone H3 tail by the Pygo-BCL9 Wnt signaling complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-21 |
Detector | ADSC CCD |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 105.720, 105.720, 51.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 106.000 - 1.700 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vpb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.197 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.370 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.120 | 1.210 |
Number of reflections | 30994 | |
<I/σ(I)> | 12.4 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.3 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | HANGING-DROP VAPOUR-DIFFUSION METHOD AT 19 CELSIUS. 26.4% PEG-3350, 200MM LISO4, 100MM TRIS PH7 |