2VOX
An oxidized tryptophan facilitates copper-binding in Methylococcus capsulatus secreted protein MopE. The structure of mercury soaked MopE to 1.9AA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 65.939, 101.282, 54.816 |
Unit cell angles | 90.00, 98.32, 90.00 |
Refinement procedure
Resolution | 41.130 - 1.900 |
R-factor | 0.222 |
Rwork | 0.221 |
R-free | 0.24500 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.022 |
RMSD bond angle | 2.347 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.100 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.550 |
Number of reflections | 27480 | |
<I/σ(I)> | 8.4 | 1.3 |
Completeness [%] | 98.2 | 97.3 |
Redundancy | 13.4 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 36-45% AMMONIUM SULPHATE, 0.1 M BISTRIS PH 7.0-7.75 |