2VOP

Crystal structure of N-terminal domains of Human La protein complexed with RNA oligomer AUUUU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-12
Detector	ADSC CCD
Spacegroup name	C 2 2 2
Unit cell lengths	96.070, 116.690, 63.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.240 - 2.800
R-factor	0.261
Rwork	0.261
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zh5
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALE
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.090	0.370
Number of reflections	8894
<I/σ(I)>	6.2	1.9
Completeness [%]	98.3	99.9
Redundancy	2.4	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1