2VN2
Crystal structure of the N-terminal domain of DnaD protein from Geobacillus kaustophilus HTA426
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9794, 0.9795, 0.9643 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 116.514, 124.707, 157.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.490 - 2.300 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.22300 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.600 |
| Data reduction software | Blu-Ice (CONTROL SOFTWARE) |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.490 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.110 | 0.370 |
| Number of reflections | 46920 | |
| <I/σ(I)> | 22.59 | 9.2 |
| Completeness [%] | 95.4 | 100 |
| Redundancy | 12.3 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
