2VN1
Crystal structure of the FK506-binding domain of Plasmodium falciparum FKBP35 in complex with FK506
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.064, 63.064, 167.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.650 - 2.350 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yat |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MrBUMP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.100 | 0.380 |
| Number of reflections | 14883 | |
| <I/σ(I)> | 41.1 | 8.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 20.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 2.7M NA MALONATE, PH5 |
