2VMK
Crystal Structure of E. coli RNase E Apoprotein - Catalytic Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-03 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.241, 75.571, 109.370 |
| Unit cell angles | 94.95, 102.03, 91.77 |
Refinement procedure
| Resolution | 47.840 - 3.300 |
| R-factor | 0.268 |
| Rwork | 0.267 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bx2 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 0.950 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.340 |
| High resolution limit [Å] | 3.220 | 3.220 |
| Rmerge | 0.110 | 0.460 |
| Number of reflections | 34917 | |
| <I/σ(I)> | 11.1 | 1.7 |
| Completeness [%] | 91.4 | 67.5 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.5 |
