2VMG

The structure of CBM51 from Clostridium perfringens GH95 in complex with methyl-galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	113
Spacegroup name	P 1 21 1
Unit cell lengths	37.475, 47.948, 38.704
Unit cell angles	90.00, 92.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.233
Rwork	0.230
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.932
Data reduction software	d*TREK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.077	0.300
Number of reflections	10010
<I/σ(I)>	8.6	2.5
Completeness [%]	96.4	93.2
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291