2VME
Structure of the wild-type discoidin II from Dictyostelium discoideum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-10 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 287.885, 56.281, 123.674 |
| Unit cell angles | 90.00, 109.10, 90.00 |
Refinement procedure
| Resolution | 26.800 - 2.450 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vm9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.556 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.820 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.090 | 0.370 |
| Number of reflections | 66709 | |
| <I/σ(I)> | 5.8 | 1.9 |
| Completeness [%] | 96.8 | 98.3 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 20% PEG 4K, 10 MM CDCL2, 100 MM HEPES PH 7.5 |
