2VLQ
F86A mutant of E9 DNase domain in complex with Im9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.058, 77.490, 41.807 |
| Unit cell angles | 90.00, 100.21, 90.00 |
Refinement procedure
| Resolution | 41.170 - 1.600 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1emv |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.707 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.170 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.340 |
| Number of reflections | 29183 | |
| <I/σ(I)> | 17.1 | 4.1 |
| Completeness [%] | 97.7 | 85.4 |
| Redundancy | 3.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.9 M AMMONIUM SULPHATE AND 100 MM BIS-TRIS PH 5.5 |
