2VLP
R54A mutant of E9 DNase domain in complex with Im9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.285, 77.920, 41.402 |
| Unit cell angles | 90.00, 100.07, 90.00 |
Refinement procedure
| Resolution | 40.820 - 2.000 |
| R-factor | 0.169 |
| Rwork | 0.166 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1emv |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.574 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.340 |
| Number of reflections | 15357 | |
| <I/σ(I)> | 18 | 5.8 |
| Completeness [%] | 98.4 | 98.4 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.9 M AMMONIUM SULPHATE AND 100 MM BIS-TRIS PH 5.5 |
