2VL4
Structural and biochemical evidence for a boat-like transition state in beta-mannosidases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2007-03-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.559, 115.038, 98.697 |
| Unit cell angles | 90.00, 112.59, 90.00 |
Refinement procedure
| Resolution | 48.910 - 1.900 |
| R-factor | 0.165 |
| Rwork | 0.162 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2je8 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.695 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.410 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.420 |
| Number of reflections | 146726 | |
| <I/σ(I)> | 11.5 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 18% PEG 3350, 0.2M NABR, 0.1M MES BUFFER PH 6.5 |
