Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.709, 203.175, 79.815 |
| Unit cell angles | 90.00, 91.81, 90.00 |
Refinement procedure
| Resolution | 48.450 - 1.950 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2G1I |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.850 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP (- AUTO MR) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.450 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.100 | 0.510 |
| Number of reflections | 155315 | |
| <I/σ(I)> | 11.5 | 2.5 |
| Completeness [%] | 86.3 | 80.6 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.45 | 281 | 50MM MES, 1MM DTT, 5MM TDP, 5MM MAGNESIUM SULFATE, 10% PEG 2000, 10% PEG 8000, 2MG KLPDC/ML, PH 6.45, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 281K |
