2VK3
Crystal structure of rat profilin 2a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 |
| Unit cell lengths | 51.100, 51.100, 67.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.450 - 1.700 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v8f |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.838 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0028) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.520 |
| Number of reflections | 18939 | |
| <I/σ(I)> | 12.7 | 2.2 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 2M AMMONIUM SULPHATE, 1M NACL, PH 4.5 |
