2VJV
Crystal structure of the IS608 transposase in complex with left end 26-mer DNA hairpin and a 6-mer DNA representing the left end cleavage site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-12 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.292, 72.407, 110.174 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.610 - 1.900 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vih |
| RMSD bond length | 0.008 |
| RMSD bond angle | 3.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.150 | 0.270 |
| Number of reflections | 38044 | |
| <I/σ(I)> | 11.3 | 4.6 |
| Completeness [%] | 89.8 | 64.2 |
| Redundancy | 3.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 6-10% PEG 600 AND 50 MM SODIUM CITRATE PH 5.0 |
