2VJN

Formyl-CoA transferase mutant variant G260A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-29
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	97.260, 97.260, 193.356
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.171
Rwork	0.169
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p5h
RMSD bond length	0.009
RMSD bond angle	1.159
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.150	0.500
Number of reflections	63094
<I/σ(I)>	7.2	2.5
Completeness [%]	99.6	99.9
Redundancy	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1