2VJI
Tailspike protein of E.coli bacteriophage HK620
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 74.216, 74.216, 175.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.380 |
| R-factor | 0.151 |
| Rwork | 0.150 |
| R-free | 0.18000 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.499 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE (RESOLVE V2.09) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.060 | 0.260 |
| Number of reflections | 115675 | |
| <I/σ(I)> | 12.7 | 5.1 |
| Completeness [%] | 97.5 | 90.2 |
| Redundancy | 4.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3 | VAPOR DIFFUSION,HANGING DROP: PROTEIN CONCENTRATION 3MG/ML,5% PEG1500,PH3.0. RESERVOIR: 10% PEG 1500, PH 3.0. DROPLET 3 MICRO LITER: 3 MICRO LITER. CRYO CONDITION:30% PEG1500. |
