2VJE

Crystal Structure of the MDM2-MDMX RING Domain Heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Collection date	2007-12-13
Spacegroup name	P 1 21 1
Unit cell lengths	54.562, 40.769, 77.514
Unit cell angles	90.00, 109.06, 90.00

Refinement procedure

Resolution	35.920 - 2.200
R-factor	0.198
Rwork	0.196
R-free	0.22300
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.011
RMSD bond angle	1.427
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.920	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.060	0.340
Number of reflections	11282
<I/σ(I)>	14.9	3.2
Completeness [%]	98.2	97.1
Redundancy	3.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		1.8 M NH4(SO4)2, 0.5 M NACL, 0.1 M NA CITRATE, PH 6.5