2VJ8

Complex of human leukotriene A4 hydrolase with a hydroxamic acid inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	67.770, 132.470, 83.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.195
Rwork	0.195
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hs6
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Refinement software	TNT (2.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800
Rmerge	0.080
Number of reflections	62521
<I/σ(I)>	5
Completeness [%]	89.1
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	6.8	298	PEG8000, IMIDAZOLE, SODIUM ACETATE,YTTERBIUM CHLORIDE, BESTATIN, PH 6.8, LIQUID DIFFUSION,TEMPERATURE 298K