2VI7

Structure of a Putative Acetyltransferase (PA1377)from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-19
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	79.709, 110.928, 134.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.300 - 2.250
R-factor	0.191
Rwork	0.191
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ge3
RMSD bond length	0.014
RMSD bond angle	1.370
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.300	2.310
High resolution limit [Å]	2.250	2.250
Rmerge	0.090	0.610
Number of reflections	28683
<I/σ(I)>	21	2.8
Completeness [%]	99.9	99.9
Redundancy	6.7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	HANGING DROP VAPOR DIFFUSION. RESERVOIR OF 500 MICROLITRES 0.1M TRIS-HCL AT PH6.5, 1.6M AMMONIUM SULFATE AND 0.1% SODIUM AZIDE. DROPS CONTAINED 1 MICROLITRE OF PROTEIN AT 13MG /ML AND AN EQUAL VOLUME OF RESERVOIR. DROPS WERE KEPT AT 291K.