2VHG
Crystal Structure of the ISHp608 Transposase in Complex with Right End 31-mer DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.466, 90.925, 93.418 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.900 |
R-factor | 0.2847 |
Rwork | 0.285 |
R-free | 0.31970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a6o |
RMSD bond length | 0.005 |
RMSD bond angle | 1.370 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.060 | 0.560 |
Number of reflections | 13127 | |
<I/σ(I)> | 25 | 3.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.2 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 15-20% PEG 3350 AND 0.1 M MES PH 6.5 |