2VGD
Crystal structure of environmental isolated GH11 in complex with xylobiose and feruloyl-arabino-xylotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-01 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 76.470, 76.470, 102.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.660 - 1.800 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APO FORM OF ENXYN11A |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.431 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.000 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.790 |
| Rmerge | 0.090 | 0.750 |
| Number of reflections | 27316 | |
| <I/σ(I)> | 20.8 | 2 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 3.75 MM FAX3 0.2 M AMMONIUM FORMATE, PH 6.6 AND 17% (W/V) POLYETHYLENE GLYCOL 3350 |
