2VFR
Alditol Oxidase from Streptomyces coelicolor A3(2): Native Enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-25 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.023, 68.614, 57.977 |
| Unit cell angles | 90.00, 95.15, 90.00 |
Refinement procedure
| Resolution | 57.740 - 1.100 |
| R-factor | 0.14 |
| Rwork | 0.139 |
| R-free | 0.16500 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.719 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.700 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.010 | 0.430 |
| Number of reflections | 167187 | |
| <I/σ(I)> | 9 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
