2VFI
Crystal structure of the Plasmodium falciparum triosephosphate isomerase in the loop closed state with 3-phosphoglycerate bound at the active site and interface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.611, 51.135, 92.185 |
| Unit cell angles | 90.00, 98.24, 90.00 |
Refinement procedure
| Resolution | 91.290 - 2.250 |
| R-factor | 0.21167 |
| Rwork | 0.209 |
| R-free | 0.26022 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o5x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.968 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.100 | 0.380 |
| Number of reflections | 22598 | |
| <I/σ(I)> | 9.2 | 2.3 |
| Completeness [%] | 95.1 | 80.5 |
| Redundancy | 2.6 | 1.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M SODIUM ACETATE PH 4.0-5.5 PEG 1450 8-24% |
