2VF7
Crystal structure of UvrA2 from Deinococcus radiodurans
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 271.710, 111.670, 103.290 |
| Unit cell angles | 90.00, 100.80, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| R-factor | 0.223 |
| Rwork | 0.219 |
| R-free | 0.29500 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.536 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.590 |
| Number of reflections | 123364 | |
| <I/σ(I)> | 7.1 | 1.1 |
| Completeness [%] | 92.1 | 62.1 |
| Redundancy | 2.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 17% PEG 3000, 0.1M CITRATE PH 5.2, 1MM TCEP |
