2VEO
X-ray structure of Candida antarctica lipase A in its closed state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 91.539, 91.539, 299.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.22100 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.937 |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.140 | 0.620 |
| Number of reflections | 63098 | |
| <I/σ(I)> | 10.2 | 2 |
| Completeness [%] | 96.0 | 85.3 |
| Redundancy | 4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 2 MICROLITER 10 MILLIGRAM/MILLILITER PROTEIN IN 0.002M TRIS-HCL, PH 8.0 MIXED WITH 1 MICROLITER 0.2M AMMONIUM SULFATE, 0.1M BIS-TRIS, PH 5.5, AND 25% (W/V) PEG 3350. |
