2VEF
Dihydropteroate synthase from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.613, 90.382, 138.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.180 - 1.800 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.622 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.210 |
| Number of reflections | 65291 | |
| <I/σ(I)> | 12.7 | 4.5 |
| Completeness [%] | 99.0 | 93.9 |
| Redundancy | 3.43 | 2.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M AMMONIUM IODIDE, 20% (W/V) POLYETHYLENE GLYCOL 3,350 |
