2VDT

Crystallographic structure of Levansucrase from Bacillus subtilis mutant S164A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-03-13
Detector	RIGAKU-MSC
Spacegroup name	P 21 21 21
Unit cell lengths	50.880, 55.800, 124.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.410 - 3.200
R-factor	0.2645
Rwork	0.265
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oyg
RMSD bond length	0.017
RMSD bond angle	2.900
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.410	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.140	0.440
Number of reflections	5627
<I/σ(I)>	9	1.8
Completeness [%]	91.7	94.9
Redundancy	2.7	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7		CRYSTALS WERE OBTAINED AFTER 12 DAYS OF MICRODIALYSIS OF THE PURIFIED PROTEIN (8 G/L) AGAINST DEIONIZED WATER., pH 7