2VDR

Integrin AlphaIIbBeta3 Headpiece Bound to a chimeric Fibrinogen Gamma chain peptide, LGGAKQRGDV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-15
Detector	CUSTOM (SBC2 3K)
Spacegroup name	P 32 2 1
Unit cell lengths	148.328, 148.328, 176.793
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.440 - 2.400
R-factor	0.15
Rwork	0.148
R-free	0.19300
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.007
RMSD bond angle	1.043
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.090	0.570
Number of reflections	609049
<I/σ(I)>	17.3	2.3
Completeness [%]	99.7	99
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	11% PEG 3350, 0.7 M MAGNESIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K