2VCP

Crystal structure of N-Wasp VC domain in complex with skeletal actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-10
Detector	ADSC CCD
Spacegroup name	P 61
Unit cell lengths	136.240, 136.240, 205.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.200
R-factor	0.275
Rwork	0.275
R-free	0.33100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a3z
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.120	0.400
Number of reflections	35056
<I/σ(I)>	8.8	2.3
Completeness [%]	98.5	95
Redundancy	3.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	277	VAPOR DIFFUSION METHOD (4 C) PROTEIN SOLUTION: 0.18MM ACTIN, 0.36MM N-WASP PEPTIDE, 5MM TRIS.HCL PH7.0, ATP 0.2MM, CACL2 0.02MM, TCEP 20MM, NAN3 0.01%. RESERVOIR: 10.2% (V/V) TACSIMATE, 13.2%(W/V)PEG 8000, 100MM HEPES PH7.0